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Understanding perception about nature is paramount to understanding human behaviour and decision making on the environment. It was performed a survey-based study in Brazil and Poland to better understand the perception of land sparing for nature and the perceived value of nature. The countries were selected by intentional sampling and given their importance for local and global biodiversity conservation, and complex socio-ecological context of conservation versus agroforestry business. It was applied an online questionnaire (N = 1030) in Brazil and face-to-face interviews in Poland (N = 322). Brazilian respondents demonstrated more pro-environmental attitudes than Polish survey participants. Regarding the question: “How much nature that is left should be spared?”, nearly 51% of Brazilians answered “everything”, compared with 13% of Polish respondents. Just under half of the respondents from Poland (45.6%) indicated that half of the nature that is left should be spared. Brazilian respondents also perceived the intrinsic value of nature to a greater extent compared with Polish respondents; in total, 76% of Brazilians respondents entirely agreed that “Nature, its plants and animals have a value on their own, independent of their usefulness for us” versus 25% of Polish respondents. Our study contributes to a better understanding of the leverage points driving pro-environmental attitudes in both countries.